Anti-CSP-C抗体(ab69876)
Key features and details
- Rabbit polyclonal to CSP-C
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-CSP-C抗体
参阅全部 CSP-C 一抗 -
描述
兔多克隆抗体to CSP-C -
宿主
Rabbit -
特异性
This antibody detects endogenous levels of total GZMH protein. -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human CSP-C (internal sequence).
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阳性对照
- A549 cells and extracts from K562 cells
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.87% Sodium chloride, 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab69876于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | ||
ICC/IF |
说明 |
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I-ELISA: 1/5000.
WB: 1/500 - 1/1000. Detects bands of approximately 21 and 54 kDa (predicted molecular weight: 27 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
靶标
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功能
Cytotoxic chymotrypsin-like serine protease with preference for bulky and aromatic residues at the P1 position and acidic residues at the P3' and P4' sites. Probably necessary for target cell lysis in cell-mediated immune responses. Participates in the antiviral response via direct cleavage of several proteins essential for viral replication. -
组织特异性
Constituvely expressed in NK cells. -
序列相似性
Belongs to the peptidase S1 family. Granzyme subfamily.
Contains 1 peptidase S1 domain. -
细胞定位
Cytoplasmic granule. Cytoplasmic granules of cytolytic T-lymphocytes. - Information by UniProt
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数据库链接
- Entrez Gene: 2999 Human
- Omim: 116831 Human
- SwissProt: P20718 Human
- Unigene: 348264 Human
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别名
- Cathepsin G like 2 antibody
- Cathepsin G-like 2 antibody
- CCP X antibody
see all
图片
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All lanes : Anti-CSP-C antibody (ab69876) at 1/500 dilution
Lane 1 : Extracts from K562 cells
Lane 2 : Extracts from K562 cells with immunizing peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 27 kDa
Observed band size: 21 kDa why is the actual band size different from the predicted?
Additional bands at: 54 kDa. We are unsure as to the identity of these extra bands. -
Immunofluorescence analysis of A549 cells using ab69876 at 1/500-1/1000 dilution.
Left image un-treated.Right image treated with immunizing peptide.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab69876 被引用在 1 文献中.
- Tang H et al. Granzyme H of cytotoxic lymphocytes is required for clearance of the hepatitis B virus through cleavage of the hepatitis B virus X protein. J Immunol 188:824-31 (2012). WB, ICC/IF ; Human . PubMed: 22156339