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Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab6721)

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Reassurance, Refunds & Replacements

If your product does not perform as described on this datasheet, we will refund or replace your product...

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Overview

Product name

Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP)
参阅全部 IgG 抗体 (1701) ...

Target species

Rabbit

Tested applications

IHC-P, WB, ELISA, Immunomicroscopy, Dot Blot, ICC/IF, IHC-Frmore details

Immunogen

Rabbit IgG, whole molecule

Properties

Form

Liquid

Storage instructions

Store at +4°C short term. Store in single use aliquots at -20°C. Avoid refreezing an aliquot. This product is stable at 4°C as an undiluted liquid. Centrifuge product prior to usage to eliminate potential protein aggregates.

Storage buffer

Preservative: 0.01% Gentamicin Sulfate
Constituents: 10mg/ml BSA, 0.15M Sodium chloride, 0.02M Potassium phosphate, pH 7.2

Concentration

Concentration information loading...

Purity

IgG fraction

Purification notes

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads.

Conjugation notes

Horseradish Peroxidase (HRP)

Clonality

Polyclonal

General notes

Many of our customers have reported seeing brown precipitates in the vials. The brown precipitates are very common with HRP conjugated antibodies; we suggest vortexing the vial and using this antibody as normal. Our customer’s feedback says the antibody worked great. If in case the antibody fails to give results then please contact our Scientific Support team for assistance.

Research areas

Secondary antibodies >> anti-Rabbit >> IgG >> Enzyme >> HRP
Immunology >> Immunoglobulins >> Heavy Chain >> IgG

  • Western blot - Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab6721)Western blot - Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab6721) image (enlarge)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab6721)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab6721) image (enlarge)

  • Immunohistochemistry (Frozen sections) - Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab6721)Immunohistochemistry (Frozen sections) - Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab6721) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab6721 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 1 Image

    IHC-P

     IHC-P: 1/1000.

    IHC-P: 1/1000.

  • ShowHide1 Image

    WB

    No star rating (7 Abreviews) See more...Read more →

    WB: 1/2000 - 1/20000. Suggested working dilution of 1/3000 (see PMID: 17222046). In addition, found to work at 1/20000 (see PMID: 16936283).

    Working dilutions are highlighted in the table below. Please note that the antibody can be diluted to 1:48,000 to 1:207,000 in many instances.

  • ELISA

     ELISA: 1/120000.

    ELISA: 1/120000.

  • ShowHide

    Immunomicroscopy

     IM: Use at an assay depe...Read more →

    IM: Use at an assay dependent dilution.

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    Dot Blot

     Dot: Use at an assay dep...Read more →

    Dot: Use at an assay dependent dilution.

  • ShowHide

    ICC/IF

     ICC/IF: 1/1000 - 1/5000. Read more →

    ICC/IF: 1/1000 - 1/5000.

  • 1 Image

    IHC-Fr

     IHC-Fr: 1/1000.

    IHC-Fr: 1/1000.

Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) images:

  Western blot - Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab6721)

Western blot - Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab6721)

Ab6721 was used with the primary antibody ab8227 in WB. See the review on ab8227.

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab6721)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab6721)

Ab6721 was used at dilution 1/100 with the primary antibody ab11370 in IHC-P. See the review on ab11370.

  Immunohistochemistry (Frozen sections) - Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab6721)

Immunohistochemistry (Frozen sections) - Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab6721)

Ab6721 was used at dilution 1/100 with the primary antibody ab35604 in IHC-Fr. See the review on ab35604.

References for Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab6721)

This product has been referenced in:

  • Kim JH  et al. Phenotypic characterization of polygenic type 2 diabetes in TALLYHO/JngJ mice. J Endocrinol 191:437-46 (2006). WB.Read more (PubMed: 17088413) »
  • Benneyworth MA  et al. Failure of NMDA receptor hypofunction to induce a pathological reduction in PV-positive GABAergic cell markers. Neurosci Lett 488:267-71 (2011). WB.Read more (PubMed: 21094213) »

See all 52 publications for this product

Publishing research using ab6721? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"