山羊Anti-Rat IgG H&L (Alexa Fluor® 488) (ab150157)

概述

  • 产品名称
    山羊Anti-Rat IgG H&L (Alexa Fluor® 488)
    参阅全部 IgG 二抗
  • 描述
    山羊多克隆抗体二抗to大鼠IgG - H&L (Alexa Fluor® 488)
  • 靶标种属
    Rat
  • 经测试应用
    适用于: IHC-Fr, Flow Cyt, IHC-P, ICC/IF, ELISAmore details
  • 偶联物
    Alexa Fluor® 488. Ex: 495nm, Em: 519nm

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 30% Glycerol, 1% BSA
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 常规说明

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to use products containing Alexa Fluor® dyes for purposes other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.

  • 研究领域

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应用

Our Abpromise guarantee covers the use of ab150157 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr Use at an assay dependent concentration.
Flow Cyt 1/2000 - 1/4000.
IHC-P Use at an assay dependent concentration.
ICC/IF 1/200 - 1/1000.
ELISA Use at an assay dependent concentration.

图片

  • ICC/IF image of ab6160 stained HeLa cells. The cells were 100% methanol fixed (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6160, 2µg/ml) overnight at +4°C. The secondary antibody (green) was ab150157 Alexa Fluor® 488 goat anti-rat IgG (H+L) used at 1µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.

  • Overlay histogram showing Jurkat cells stained with ab30446 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab30446, 0.01μg/1x106 cells) for 30 min at 22°C. The secondary antibody Goat anti-rat IgG H&L (Alexa Fluor® 488) (ab150157) was used at 1/4000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rat IgG2b [RTK4530] (ab18541, 0.01μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

文献

This product has been referenced in:
  • Xiang Y  et al. Effect of Yi Guan Jian decoction on differentiation of bone marrow mesenchymalstem cells into hepatocyte-like cells in dimethylnitrosamine-induced liver cirrhosis in mice. Mol Med Rep 15:613-626 (2017). Read more (PubMed: 28035356) »
  • Kornicka K  et al. The effects of the DNA methyltranfserases inhibitor 5-Azacitidine on ageing, oxidative stress and DNA methylation of adipose derived stem cells. J Cell Mol Med 21:387-401 (2017). Read more (PubMed: 27998022) »

See all 10 Publications for this product

客户评价及客户问答

Application
Immunocytochemistry/ Immunofluorescence
Asynchronous HeLa cells were paraformaldehyde-fixed, permeablized, and immunofluorescently labeled with an anti-histone H3 (phospho S10) antibody, which is expected to stain only mitotic cells. The fluorescent secondary antibody (ab150157) exhibited the correct affinity for the primary antibody with no apparent background or non-specific staining. In the "Merge" image below, the DAPI is pseudocolored red, while ab150157 is pseudocolored green.
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Dr. Kirk Mcmanus

Verified customer

提交于 Oct 18 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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