Application
Western blot
Experimental conditions:
Bone tissue lysate (tibia) extracted with RIPA buffer
10% SDS-PAGE
Blocking 1h with 5% milk in TBS-T at RT
Incubation with primary AB 1:1000 overnight at 4ºC
Incubation with secondary AB (ab6721 - Goat Anti-Rabbit IgG) 1:2000 for 1h at RT
Developed with ECL for 537s
Results
No nonspecific binding. No background. Clear bands.
Bone tissue lysate (tibia) extracted with RIPA buffer
10% SDS-PAGE
Blocking 1h with 5% milk in TBS-T at RT
Incubation with primary AB 1:1000 overnight at 4ºC
Incubation with secondary AB (ab6721 - Goat Anti-Rabbit IgG) 1:2000 for 1h at RT
Developed with ECL for 537s
Results
No nonspecific binding. No background. Clear bands.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Helder Fonseca
Verified customer
提交于 Oct 07 2014