山羊抗兔IgG H&L (Cy5 ®)预吸附二抗(ab97077)
Key features and details
- Goat Anti-Rabbit IgG H&L (Cy5 ®) preadsorbed
- Conjugation: Cy5 ®. Ex: 650nm, Em: 667nm
- Host species: Goat
- Isotype: IgG
- Suitable for: WB, ICC/IF, Flow Cyt, IHC-P
概述
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产品名称
山羊抗兔IgG H&L (Cy5 ®)预吸附二抗
参阅全部 IgG 二抗 -
宿主
Goat -
靶标种属
Rabbit -
特异性
By immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Reduced cross-reactivity to bovine, chicken, goat, horse, human, mouse, pig and rat IgG was detected.
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经测试应用
适用于: WB, ICC/IF, Flow Cyt, IHC-Pmore details -
最小交叉反应
Chicken, Cow, Horse, Human, Mouse, Pig, Rat more details -
偶联物
Cy5 ®. Ex: 650nm, Em: 667nm
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.2% BSA, PBS -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
Antiserum was cross absorbed using bovine, chicken, horse, human, mouse, pig and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Cy5 ®. -
克隆
多克隆 -
同种型
IgG -
研究领域
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab97077于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000 - 1/5000. Predicted molecular weight: 36 kDa.
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ICC/IF |
1/50 - 1/500.
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Flow Cyt |
1/100 - 1/1000.
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IHC-P |
1/50 - 1/500.
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说明 |
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WB
1/1000 - 1/5000. Predicted molecular weight: 36 kDa. |
ICC/IF
1/50 - 1/500. |
Flow Cyt
1/100 - 1/1000. |
IHC-P
1/50 - 1/500. |
图片
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Rab7 and LAMP1 are highly colocalized in BS-C-1 cells and in HeLa cells.
Rab7 was detected with an Rabbit anti-Rab7 antibody, followed by ab97077 at 1/500 dilution in ICC/IF analysis of HeLa cells.
(From Figure S1 of Humphries et al)
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Sample: mouse embryonic stem cell-differentiated embryoid bodies (EBs)
Preparation:- Fix in 3% PFA in PBS for 30 min at RT
- Incubate in 7.5% sucrose-PBS for 3h at RT
- Incubate in 15% sucrose-PBS at 4 degree Celsius overnight
- Embed the EBs in tissue-Tek OCT compound
- Cut frozen sections to 4-20 µm thickness
Primary antibody: Rabbit anti-laminin alpha 1, 1:400
Secondary antibody:Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Cy5®) (ab97077), 1:100
F-actin was stained with CytoPainter F-actin staining kit (blue) (ab112124), 1:100
Nuclei were counterstained with SYTO 16 -
ab97077 (1/200) detecting ab5176 in assynchronous HeLa cells (red). Cells were fixed in paraformaldehyde, permeabilised in 0.5% Triton X-100/PBS and counterstained with DAPI in order to highlight the nucleus (blue); ab5176, a rabbit polyclonal to Histone H3 (phospho S10), was used at 1/4000 dilution.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (34)
ab97077 被引用在 34 文献中.
- Yoshida Y et al. Differing impact of phosphoglycerate mutase 1-deficiency on brown and white adipose tissue. iScience 25:104268 (2022). PubMed: 35521515
- Hayashi Y et al. Coagulation factors promote brown adipose tissue dysfunction and abnormal systemic metabolism in obesity. iScience 25:104547 (2022). PubMed: 35754738
- Yoshida Y et al. Brown adipose tissue dysfunction promotes heart failure via a trimethylamine N-oxide-dependent mechanism. Sci Rep 12:14883 (2022). PubMed: 36050466
- Schmiderer L et al. Site-specific CRISPR-based mitochondrial DNA manipulation is limited by gRNA import. Sci Rep 12:18687 (2022). PubMed: 36333335
- Bouchard G et al. Multiomics Analysis of Spatially Distinct Stromal Cells Reveals Tumor-Induced O-Glycosylation of the CDK4-pRB Axis in Fibroblasts at the Invasive Tumor Edge. Cancer Res 82:648-664 (2022). PubMed: 34853070