山羊抗兔IgG H&L (Cy3 ®)预吸附二抗(ab97075)
Key features and details
- Goat Anti-Rabbit IgG H&L (Cy3 ®) preadsorbed
- Conjugation: Cy3 ®. Ex: 552nm, Em: 565nm
- Host species: Goat
- Isotype: IgG
- Suitable for: IHC-P, ICC/IF, Flow Cyt
概述
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产品名称
山羊抗兔IgG H&L (Cy3 ®)预吸附二抗
参阅全部 IgG 二抗 -
宿主
Goat -
靶标种属
Rabbit -
特异性
By immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Reduced cross-reactivity to bovine, chicken, goat, horse, human, mouse, pig and rat IgG was detected.
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经测试应用
适用于: IHC-P, ICC/IF, Flow Cytmore details -
最小交叉反应
Chicken, Cow, Horse, Human, Mouse, Pig, Rat more details -
免疫原
Other Immunogen Type corresponding to Rabbit IgG.
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偶联物
Cy3 ®. Ex: 552nm, Em: 565nm
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.2% BSA, PBS -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
Antiserum was cross absorbed using bovine, chicken, horse, human, mouse, pig and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Cy3 ®. -
克隆
多克隆 -
同种型
IgG -
研究领域
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab97075于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
1/50 - 1/500.
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ICC/IF |
1/50 - 1/500.
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Flow Cyt |
1/100 - 1/1000.
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说明 |
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IHC-P
1/50 - 1/500. |
ICC/IF
1/50 - 1/500. |
Flow Cyt
1/100 - 1/1000. |
图片
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Sample: mouse embryonic stem cell-differentiated embryoid bodies (EBs)
Preparation:
Fix in 3%PFA in PBS for 30 min at RT
Incubate in 7.5% sucrose-PBS for 3h at RT
Incubate in 15% sucrose-PBS at 4 degree Celsius overnight
Embed the EBs in tissue-Tek OCT compound
Cut frozen sections to 4-20 µm thicknessPrimary antibody: Rabbit anti-laminin alpha 1, 1:400
Secondary antibody:Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Cy3®) (ab97075), 1:100
Nuclei were counterstained with SYTO 16 -
Image: Courtesy of Dr. Shaohua Li, UMDNJ-Robert Wood Johnson Medical School
Sample: mouse endoderm cell
Preparation:
Fix in 3% PFA in PBS for 30 min at RT
Primary antibody: Rabbit anti-talin (ab71333), 1:100
Secondary antibody: Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Cy3® 488) preadsorbed (ab97075), 1:100
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (39)
ab97075 被引用在 39 文献中.
- Júnior C et al. Multi-Step Extracellular Matrix Remodelling and Stiffening in the Development of Idiopathic Pulmonary Fibrosis. Int J Mol Sci 24:N/A (2023). PubMed: 36675222
- Yabuno S et al. Synergistic therapeutic effects of intracerebral transplantation of human modified bone marrow-derived stromal cells (SB623) and voluntary exercise with running wheel in a rat model of ischemic stroke. Stem Cell Res Ther 14:10 (2023). PubMed: 36691091
- Lu T et al. Gut Microbiota-Derived Glutamine Attenuates Liver Ischemia/Reperfusion Injury via Macrophage Metabolic Reprogramming. Cell Mol Gastroenterol Hepatol 15:1255-1275 (2023). PubMed: 36706918
- Zhang H et al. Macrophage migration inhibitory factor facilitates astrocytic production of the CCL2 chemokine following spinal cord injury. Neural Regen Res 18:1802-1808 (2023). PubMed: 36751809
- Narciso M et al. Lung Micrometastases Display ECM Depletion and Softening While Macrometastases Are 30-Fold Stiffer and Enriched in Fibronectin. Cancers (Basel) 15:N/A (2023). PubMed: 37190331