山羊抗兔IgG H&L (Biotin)预吸附二抗(ab97073)
Key features and details
- Goat Anti-Rabbit IgG H&L (Biotin) preadsorbed
- Conjugation: Biotin
- Host species: Goat
- Isotype: IgG
- Suitable for: IHC-P, ICC/IF, ELISA, WB
概述
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产品名称
山羊抗兔IgG H&L (Biotin)预吸附二抗
参阅全部 IgG 二抗 -
宿主
Goat -
靶标种属
Rabbit -
特异性
By immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Reduced cross-reactivity to bovine, chicken, goat, horse, human, mouse, pig and rat IgG was detected.
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经测试应用
适用于: IHC-P, ICC/IF, ELISA, WBmore details -
最小交叉反应
Chicken, Cow, Horse, Human, Mouse, Pig, Rat more details -
偶联物
Biotin
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.09% Sodium azide
Constituents: 0.2% BSA, PBS -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
Antiserum was cross absorbed using bovine, chicken, horse, human, mouse, pig and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Biotin. Biotinylated antibody was demonstrated with avidin/peroxidase when coated on microtiter wells. -
克隆
多克隆 -
同种型
IgG -
研究领域
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab97073于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
1/250 - 1/2500.
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ICC/IF |
1/100 - 1/500.
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ELISA |
1/10000 - 1/200000. (Primary)
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WB |
1/10000 - 1/200000. (Chemiluminescent)
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说明 |
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IHC-P
1/250 - 1/2500. |
ICC/IF
1/100 - 1/500. |
ELISA
1/10000 - 1/200000. (Primary) |
WB
1/10000 - 1/200000. (Chemiluminescent) |
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (6)
ab97073 被引用在 6 文献中.
- Katsur M et al. Exosomes from neuronal stem cells may protect the heart from ischaemia/reperfusion injury via JAK1/2 and gp130. J Cell Mol Med 25:4455-4465 (2021). PubMed: 33797200
- Iso-O N et al. Malaria Parasites Hijack Host Receptors From Exosomes to Capture Lipoproteins. Front Cell Dev Biol 9:749153 (2021). PubMed: 34858976
- Takov K et al. Comparison of small extracellular vesicles isolated from plasma by ultracentrifugation or size-exclusion chromatography: yield, purity and functional potential. J Extracell Vesicles 8:1560809 (2019). PubMed: 30651940
- Takov K et al. Confounding factors in vesicle uptake studies using fluorescent lipophilic membrane dyes. J Extracell Vesicles 6:1388731 (2017). PubMed: 29184625
- Zhang W et al. miR-577 inhibits glioblastoma tumor growth via the Wnt signaling pathway. Mol Carcinog 55:575-85 (2016). PubMed: 25764520
- Charalambous C et al. Novel spatiotemporal glycome changes in the murine placenta during placentation based on BS-I lectin binding patterns. Anat Rec (Hoboken) 296:921-32 (2013). PubMed: 23580480