Application
Western blot
This antibody is super specific! It does not react with mouse IgG2a or human IgG, or any IgG light chain I tested so far.
I use it because I work with mouse tumor lysate in western blot. All tumor lysate contains blood, so IgG is always in the lysates. When my protein of interest is at the same molecular weight of IgG light chain, I use this antibody.
The purpose of western blot I show here is to verify if this antibody reacts with light chain of mouse IgG1 specifically, so there is no primary antibody used.
The antibody is quite clean. The image I show is over-exposed with some background in order to confirm there is absolutely no IgG light chain signal.
I use it because I work with mouse tumor lysate in western blot. All tumor lysate contains blood, so IgG is always in the lysates. When my protein of interest is at the same molecular weight of IgG light chain, I use this antibody.
The purpose of western blot I show here is to verify if this antibody reacts with light chain of mouse IgG1 specifically, so there is no primary antibody used.
The antibody is quite clean. The image I show is over-exposed with some background in order to confirm there is absolutely no IgG light chain signal.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Oct 14 2015