山羊抗小鼠IgG H&L (FITC) (ab97022)
Key features and details
- Goat Anti-Mouse IgG H&L (FITC)
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC/IF, IHC-P, Flow Cyt
概述
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产品名称
山羊抗小鼠IgG H&L (FITC)
参阅全部 IgG 二抗 -
宿主
Goat -
靶标种属
Mouse -
特异性
By immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgG and with light chains common to other Mouse immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. -
经测试应用
适用于: ICC/IF, IHC-P, Flow Cytmore details -
偶联物
FITC. Ex: 493nm, Em: 528nm
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.2% BSA, PBS -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to FITC. -
共轭说明
F/P ratio is 5.1 -
克隆
多克隆 -
同种型
IgG -
研究领域
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab97022于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
ICC/IF |
1/50 - 1/500.
|
|
IHC-P |
1/50 - 1/500.
|
|
Flow Cyt |
1/50 - 1/200.
|
说明 |
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ICC/IF
1/50 - 1/500. |
IHC-P
1/50 - 1/500. |
Flow Cyt
1/50 - 1/200. |
图片
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Sample: mouse embryonic stem cell-differentiated embryoid bodies (EBs)
Preparation:- Fix in 3%PFA in PBS for 30 min at RT
- Incubate in 7.5% sucrose-PBS for 3h at RT
- Incubate in 15% sucrose-PBS at 4 degree Celsius overnight
- Embed the EBs in tissue-Tek OCT compound
- Cut frozen sections to 4-20 µm thickness
Primary antibody: Mouse anti-E-cadherin, 1:100
Secondary antibody:Goat polyclonal Secondary Antibody to Mouse IgG - H&L (FITC) (ab97022), 1:100
Nuclei were counterstained with DAPI
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (36)
ab97022 被引用在 36 文献中.
- Mohseni F et al. Berberine hydrochloride improves cognitive deficiency through hippocampal up-regulation of neurotrophins following inhalant self-administration of methamphetamine. Iran J Basic Med Sci 26:23-29 (2023). PubMed: 36594063
- Rafaiee R et al. Bone marrow mesenchymal stem cells improve cognitive impairments induced by methamphetamine in rats and reduce relapse. Bioimpacts 13:97-108 (2023). PubMed: 37193077
- English AM & Moon SL Measuring Bulk Translation Activity in Single Mammalian Cells During the Integrated Stress Response. Methods Mol Biol 2428:63-73 (2022). PubMed: 35171473
- Allahmoradi H et al. Anti-tumor and anti-metastatic activity of the FGF2 118-126 fragment dependent on the loop structure. Biochem J 479:1285-1302 (2022). PubMed: 35638868
- Burke JM et al. RNase L activation in the cytoplasm induces aberrant processing of mRNAs in the nucleus. PLoS Pathog 18:e1010930 (2022). PubMed: 36318584