山羊F(ab')2抗小鼠IgM mu chain (FITC) (ab5926)
Key features and details
- Goat F(ab')2 Anti-Mouse IgM mu chain (FITC)
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC/IF, Flow Cyt, ELISA
概述
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产品名称
山羊F(ab')2抗小鼠IgM mu chain (FITC)
参阅全部 IgM 二抗 -
宿主
Goat -
靶标种属
Mouse -
特异性
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Mouse IgM and Mouse Serum. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c). Specificity was confirmed by ELISA at less than 1% cross reactivity against other mouse heavy or light chain isotypes. -
经测试应用
适用于: ICC/IF, Flow Cyt, ELISAmore details -
免疫原
Mouse IgM mu chain
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偶联物
FITC. Ex: 493nm, Em: 528nm
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 0.3% Potassium phosphate, 1% BSA -
Concentration information loading...
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纯化说明
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. -
共轭说明
Fluorescein isothiocyanate (FITC) (Molecular Weight 390 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 2.6 moles FITC per mole of Goat IgG F(ab’)2 -
克隆
多克隆 -
同种型
IgG -
研究领域
应用
应用 | Ab评论 | 说明 |
---|---|---|
ICC/IF |
1/1000 - 1/5000.
|
|
Flow Cyt |
1/500 - 1/2500.
|
|
ELISA |
1/10000 - 1/50000.
|
说明 |
---|
ICC/IF
1/1000 - 1/5000. |
Flow Cyt
1/500 - 1/2500. |
ELISA
1/10000 - 1/50000. |
图片
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab5926 被引用在 2 文献中.
- Zhong Y et al. Spheres derived from the human SK-RC-42 renal cell carcinoma cell line are enriched in cancer stem cells. Cancer Lett 299:150-60 (2010). Flow Cyt . PubMed: 20846785
- Wang K et al. Dynamic epigenetic regulation of the Oct4 and Nanog regulatory regions during neural differentiation in rhesus nuclear transfer embryonic stem cells. Cloning Stem Cells 11:483-96 (2009). ICC/IF ; Rhesus monkey, Human . PubMed: 20025521