重组Anti-Glycogen synthase 1/GYS1抗体[EP817Y] (ab40810)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP817Y] to Glycogen synthase 1/GYS1
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Glycogen synthase 1/GYS1抗体[EP817Y]
参阅全部 Glycogen synthase 1/GYS1 一抗 -
描述
兔单克隆抗体[EP817Y] to Glycogen synthase 1/GYS1 -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: Flow Cyt (Intra) or ICC/IF -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide within Human Glycogen synthase 1/GYS1 aa 700-800 (C terminal). The exact sequence is proprietary.
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阳性对照
- WB: HeLa whole cell lysate (ab150035), HEK293 cell lysate.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.05% BSA, 49% PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP817Y -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab40810于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | (3) |
1/10000 - 1/50000. Detects a band of approximately 85 kDa (predicted molecular weight: 81 kDa).
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说明 |
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WB
1/10000 - 1/50000. Detects a band of approximately 85 kDa (predicted molecular weight: 81 kDa). |
靶标
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功能
Transfers the glycosyl residue from UDP-Glc to the non-reducing end of alpha-1,4-glucan. -
通路
Glycan biosynthesis; glycogen biosynthesis. -
疾病相关
Defects in GYS1 are the cause of muscle glycogen storage disease type 0 (GSD0b) [MIM:611556]; also known as muscle glycogen synthase deficiency. GSD0b is a metabolic disorder characterized by fasting hypoglycemia presenting in infancy or early childhood. The role of muscle glycogen is to provide critical energy during bursts of activity and sustained muscle work. -
序列相似性
Belongs to the glycosyltransferase 3 family. - Information by UniProt
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数据库链接
- Entrez Gene: 2997 Human
- Entrez Gene: 14936 Mouse
- Entrez Gene: 690987 Rat
- Omim: 138570 Human
- SwissProt: P13807 Human
- SwissProt: Q9Z1E4 Mouse
- SwissProt: A2RRU1 Rat
- Unigene: 386225 Human
see all -
别名
- Glycogen [starch] synthase antibody
- Glycogen synthase 1 (muscle) antibody
- Glycogen synthase 1 antibody
see all
图片
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All lanes : Anti-Glycogen synthase 1/GYS1 antibody [EP817Y] (ab40810) at 1/10000 dilution
Lane 1 : Wild-type HeLa lysate
Lane 2 : Glycogen synthase 1/GYS1 knockout HeLa lysate
Lane 3 : HEK-293 lysate
Lane 4 : Daudi lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 81 kDaLanes 1-4: Merged signal (red and green). Green - ab40810 observed at 80 kDa. Red - loading control ab8245 observed at 37 kDa.
ab40810 Recombinant Anti-Glycogen synthase 1/GYS1 antibody [EP817Y] was shown to specifically react with Glycogen synthase 1/GYS1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265388 (knockout cell lysate ab257462) was used. Wild-type and Glycogen synthase 1/GYS1 knockout samples were subjected to SDS-PAGE. ab40810 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 10000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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Anti-Glycogen synthase 1/GYS1 antibody [EP817Y] (ab40810) at 1/50000 dilution + HeLa cell lysate at 10 µg
Predicted band size: 81 kDa
Observed band size: 85 kDa why is the actual band size different from the predicted?
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (19)
ab40810 被引用在 19 文献中.
- Hu MM et al. Sesamol counteracts on metabolic disorders of middle-aged alimentary obese mice through regulating skeletal muscle glucose and lipid metabolism. Food Nutr Res 66:N/A (2022). PubMed: 35382382
- Ma YD et al. The Effects of Altered Endometrial Glucose Homeostasis on Embryo Implantation in Type 2 Diabetic Mice. Reprod Sci 28:703-714 (2021). PubMed: 33151524
- Zheng L et al. High-Intensity Interval Training Restores Glycolipid Metabolism and Mitochondrial Function in Skeletal Muscle of Mice With Type 2 Diabetes. Front Endocrinol (Lausanne) 11:561 (2020). PubMed: 32922365
- Shen J et al. Pressure suppresses hepatocellular glycogen synthesis through activating the p53/Pten pathway. Mol Med Rep 19:5105-5114 (2019). PubMed: 31059076
- Kakhlon O et al. Guaiacol as a drug candidate for treating adult polyglucosan body disease. JCI Insight 3:N/A (2018). PubMed: 30185673