Application
Western blot
Sample
Mouse Tissue lysate - whole (B16 tumor lysate)
Gel Running Conditions
Reduced Denaturing (4-20% TG gels)
Loading amount
50 µg
Specification
B16 tumor lysate
Blocking step
Milk as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk In TBST
Dilution
1/10000
Secondary antibody
Name
Non-Abcam antibody was used: Anti-rabbit IGG HRP 7074 cell signaling
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL with licor c-digit
Exposure
6 minute(s) and 0 second(s)
Positive control
human samples on same gel gave positive signal
Additional data
Abcam response
Thank you for sharing your feedback. We have validated this antibody in mouse samples using Western Blotting, and are happy to provide a refund, credit or replacement if the antibody is not working as it should under our Abpromise guarantee.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Apr 10 2018