Anti-Glutathione S Transferase alpha 1抗体(ab96043)

概述

  • 产品名称
    Anti-Glutathione S Transferase alpha 1抗体
    参阅全部 Glutathione S Transferase alpha 1 一抗
  • 描述
    兔多克隆抗体to Glutathione S Transferase alpha 1
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Rat, Human
    预测可用于: Mouse
  • 免疫原

    Synthetic peptide corresponding to Human Glutathione S Transferase alpha 1 aa 50-150 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab107709)

  • 阳性对照
    • This antibody gave a positive signal in both Human and Rat Liver tissue lysates.

性能

应用

Our Abpromise guarantee covers the use of ab96043 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).

靶标

  • 功能
    Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.
  • 组织特异性
    Liver.
  • 序列相似性
    Belongs to the GST superfamily. Alpha family.
    Contains 1 GST C-terminal domain.
    Contains 1 GST N-terminal domain.
  • 结构域
    The C-terminal domain may form a component of the hydrophobic substrate-binding site, but in contrast appears not to be directly involved in GSH binding and is not absolutely essential for catalytic activity.
  • 细胞定位
    Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Glutathione S alkyltransferase A1 antibody
    • Glutathione S aryltransferase A1 antibody
    • Glutathione S transferase 2 antibody
    • Glutathione S transferase A1 antibody
    • Glutathione S transferase alpha 1 antibody
    • Glutathione S transferase Ha subunit 1 antibody
    • Glutathione S-transferase A1 antibody
    • GST class-alpha member 1 antibody
    • GST epsilon antibody
    • GST HA subunit 1 antibody
    • GST, class alpha, 1 antibody
    • GST-epsilon antibody
    • GST2 antibody
    • GSTA1 1 antibody
    • GSTA1 antibody
    • GSTA1-1 antibody
    • GSTA1_HUMAN antibody
    • GTH1 antibody
    • HA subunit 1 antibody
    • MGC131939 antibody
    • OTTHUMP00000016611 antibody
    • S (hydroxyalkyl)glutathione lyase A1 antibody
    see all

图片

  • All lanes : Anti-Glutathione S Transferase alpha 1 antibody (ab96043) at 1 µg/ml

    Lane 1 : Human liver tissue lysate - total protein (ab29889)
    Lane 2 : Liver (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 26 kDa
    Observed band size : 26 kDa
    Additional bands at : 45 kDa,52 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 2 minutes
  • All lanes : Anti-Glutathione S Transferase alpha 1 antibody (ab96043) at 1 µg/ml

    Lane 1 : Recombinant Human Glutathione S Transferase alpha 1 protein (ab95381) at 0.1 µg
    Lane 2 : Recombinant Human Glutathione S Transferase alpha 1 protein (ab95381) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 26 kDa


    Exposure time : 8 minutes

实验方案

文献

This product has been referenced in:
  • Civantos E  et al. Sitagliptin ameliorates oxidative stress in experimental diabetic nephropathy by diminishing the miR-200a/Keap-1/Nrf2 antioxidant pathway. Diabetes Metab Syndr Obes 10:207-222 (2017). WB ; Rat . Read more (PubMed: 28652790) »

See 1 Publication for this product

客户评价及客户问答

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Zebrafish Cell (Whole mount Immunofluorescence)
Permeabilization
Yes - Methanol
Specification
Whole mount Immunofluorescence
Blocking step
2% BSA + 10 % Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative
Paraformaldehyde
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提交于 Feb 20 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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