Application
Western blot
Sample
Human Cell lysate - whole cell (Human Lens epithelial cells)
Loading amount
25 µg
Specification
Human Lens epithelial cells
Gel Running Conditions
Reduced Denaturing (12% SDS-PAGE)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Dilution
1/1000
Incubation time
20 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 0.1% Tween 20-TBS
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-rabbit IgG-HRP conjugated
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/7000
Detection
Detection method
ECL+
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 12 kDa
Positive control
My sample
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Dec 22 2011