Application
Immunohistochemistry (Frozen sections)
Sample
Apteronotus leptorhynchus Tissue sections (Brain, spinal cord)
Specification
Brain, spinal cord
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.3% Triton X-100
Blocking step
3% sheep serum, 1% BSA, 1% teleostean gelatine in TBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C
Other product details
Dilution
1/100
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: blocking solution
Secondary antibody
Name
Non-Abcam antibody was used: Alexa Fluor 488 - conjugated goat anti-rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/200
Additional data
Additional Notes
The antibody was applied on 16 µm thick sections of brown ghost knifefish spinal cord and brain. 2% paraformaldehyde was used during perfusion and for post-fixation of the tissue before cryosectioning. No specific staining could be detected in either tissue sample.
Abcam response
Thank you very much for testing this antibody in Apteronotus. We are sorry that it apparently does not work with this species under the given conditions.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Ruxandra Sirbulescu
Verified customer
提交于 Nov 14 2011