Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Cerebellum)
Specification
Cerebellum
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: rt°C
Other product details
Dilution
1/1000
Incubation time
2 hour(s) and 0 minute(s) · Diluent: TBS/BSA/azide
Secondary antibody
Name
Non-Abcam antibody was used: anti Rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/200
Additional data
Additional Notes
Very nice. I can see two very good examples of the bulbous endfeet of the primary Bergmann glia primary processes connecting with the inner Pial membrane.
NB: In the submitted image, it seems that these Glia are shown to be extensively ramified, are they "mimicking" the primary/secondary processes of the Purkinje cells?
NB: In the submitted image, it seems that these Glia are shown to be extensively ramified, are they "mimicking" the primary/secondary processes of the Purkinje cells?
Abcam response
Many thanks for your feedback on ab73593 (Glutamine Synthetase antibody) in IHC-P on rat brain tissue. This is the first report of this antibody's performace in IHC-P and we welcome comments on the staining observed in this abreview.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Carl Hobbs
Verified customer
提交于 May 19 2010