Application
Western blot
Sample
Apteronotus leptorhynchus Tissue lysate - whole (Brain)
Loading amount
50 µg
Specification
Brain
Gel Running Conditions
Reduced Denaturing (4-20%)
Blocking step
Milk as blocking agent for 15 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Other product details
Dilution
1/700
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 24°C · Diluent: 2.5% milk in PBS-T
Secondary antibody
Name
Non-Abcam antibody was used: Alexa488-conjugated goat anti-mouse IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/1000
Detection
Detection method
fluorescence scanning
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 42 kDa Non-specific: 30, 18 kDa
Additional data
Additional Notes
The antibody identifies a band at the predicted molecular weight, however numerous other bands are also detected, potentially degradation products.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Jul 03 2012