Application
Western blot
Sample
Mouse Cell lysate - whole cell (Isolated Hippocampal neurons (E16))
Loading amount
100000 cells
Specification
Isolated Hippocampal neurons (E16)
Gel Running Conditions
Reduced Denaturing (15% SDS-PAGE Tris-Glycine)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/1000
Incubation time
1 hour(s) and 30 minute(s) · Temperature: 25°C
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-Rabbit-HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000
Detection
Detection method
ECL+
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 0 kDa Non-specific: 36 kDa
Positive control
Rabbit anti-GLUT3 (AB1344-Millipore)
Additional data
Additional Notes
GLUT-3 is expressed in neurons and astrocytes. Although the predicted MW of mouse GLUT-3 is 53.5 kDa, its actual MW is around 60 kDa (most likely due to post-translational modification) (PMID: 10702264)
Abcam response
Our Scientific Support team is currently investigating this negative Abreview, and we will resolve the issue in accordance with our Abpromise.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Gregory O'Sullivan
Verified customer
提交于 Jun 14 2012