Glucose Assay试剂盒(ab65333)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric/Fluorometric
- Platform: Microplate reader
- Assay time: 40 min
- Sample type: Cell culture supernatant, Cell Lysate, Other biological fluids, Plasma, Serum, Tissue Lysate, Urine
- Sensitivity: 1 µM
概述
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产品名称
Glucose Assay试剂盒
参阅全部 Glucose 试剂盒 -
检测方法
Colorimetric/Fluorometric -
样品类型
Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Cell Lysate, Tissue Lysate -
检测类型
Quantitative -
灵敏度
1 µM -
范围
1 µM - 10000 µM -
检测时间
0h 40m -
产品概述
Glucose Assay Kit ab65333 is a rapid, simple and sensitive assay used to quantify glucose levels in biological samples such as serum, plasma, and other body fluids, food, growth medium, etc.
In the glucose assay protocol, the glucose enzyme mix oxidizes glucose to generate a product which reacts with a dye to generate color (λ = 570 nm) and fluorescence (Ex/Em = 535/587 nm). The generated color and fluorescence is proportionally to the amount of glucose.
The kit detects glucose in the range 1-10000 µM.
Glucose assay protocol summary:
- add samples (deproteinized) and standards to wells
- add reaction mix and incubate for 30 min at 37ºC
- analyze with microplate reader -
说明
This product is manufactured by BioVision, an Abcam company and was previously called K606 Glucose Colorimetric/Fluorometric Assay Kit. K606-100 is the same size as the 100 test size of ab65333.
If you have reducing substances in your samples, we recommend using Glucose Detection Kit II (ab102517).
Review our Metabolism Assay Guide to learn about assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also about how to assay metabolic function in live cells using your plate reader.
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平台
Microplate reader
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 100 tests 2000 tests Assay Buffer II 1 x 25ml 20 x 25ml Development Enzyme Mix II 1 vial 20 vials OxiRed Probe 1 x 200µl 20 x 200µl Glucose Standard 1 x 100µl 20 x 100µl -
研究领域
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相关性
Glucose (C6H12O6; FW: 180.16) is a ubiquitous energy source in most organisms, from bacteria to humans. The breakdown of carbohydrates produces mono- and disaccharides, most of which is glucose. Through glycolysis and TCA (citric acid cycle), glucose is oxidized to eventually form CO2 and water, generating the universal energy molecule ATP. Glucose is a primary source of energy for the brain and a critical component in the production of proteins and in lipid metabolism and therefore measurement of glucose level is a key diagnostic parameter for many metabolic disorders.
图片
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Shao et al investigated the functional outcome of long- term curcumin supplementation on glucose homeostasis. Glucose metabolism was determined in animals with low fat diet (LFD), high fat diet (HFD) and HFD with curcumin feeding using ab65333. Intraperitoneal insulin tolerance tests(IPITT) were conducted at the end of the 26 weeks. It was concluded curcumin improves insulin sensitivity and disposal of glucose.
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Standard curve: mean of duplicates (+/- SD) with background reads subtracted
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Standard curve: mean of duplicates (+/- SD) with background reads subtracted
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Glucose measured in cell lysates showing quantity (nmol) per million cells.
Samples with the concentration of 2x107 cells/mL were used. Samples were diluted 1.5-13.5 fold and measured colorimetrically.
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Glucose measured in human biological fluids showing quantity (µmol) per mL of tested sample. Samples were diluted 13.5 fold and measured colorimetrically.
数据表及文件
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SDS download
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Datasheet download
文献 (195)
ab65333 被引用在 195 文献中.
- Zhou Y et al. Glutamate dehydrogenase 1 mediated glutaminolysis sustains HCC cells survival under glucose deprivation. J Cancer 13:1061-1072 (2022). PubMed: 35154470
- Tsai SC et al. Anti-Obesity Effect of Nostoc commune Ethanol Extract In Vitro and In Vivo. Nutrients 14:N/A (2022). PubMed: 35267941
- Wünsch D et al. Luciferase-Based Determination of ATP/NAD(H) Pools in a Marine (Environmental) Bacterium. Microb Physiol 32:122-134 (2022). PubMed: 35340012
- Morales-Lange B et al. Dietary Inclusion of Hydrolyzed Debaryomyces hansenii Yeasts Modulates Physiological Responses in Plasma and Immune Organs of Atlantic Salmon (Salmo salar) Parr Exposed to Acute Hypoxia Stress. Front Physiol 13:836810 (2022). PubMed: 35418880
- Jiang C et al. Effect of Particle Size on Physicochemical Properties and in vitro Hypoglycemic Ability of Insoluble Dietary Fiber From Corn Bran. Front Nutr 9:951821 (2022). PubMed: 35911126