Application
Western blot
Sample
Cow Tissue lysate - whole (Bovine Coronary Artery)
Loading amount
35 µg
Specification
Bovine Coronary Artery
Gel Running Conditions
Reduced Denaturing (9%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1X TBST
Secondary antibody
Name
Non-Abcam antibody was used: goat anti-mouse IgG-HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
Chemiluminescence
Exposure
20 second(s)
Bands
Specific: 58 kDa Non-specific: N/A kDa
Additional data
Additional Notes
Lane 1: Rat A7r5 cell lysate
Lane 2: Bovine Coronary Artery tissue lysate
Lane 2: Bovine Coronary Artery tissue lysate
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
hirotaka ata
Verified customer
提交于 Apr 01 2010