Methanol lysis holds advantages over both NP-40 and mechanical lysis in that it much quicker, requiring only 2 minutes incubation at room temperature, followed by centrifugation and aspiration of the supernatant. Please find attached data from my experiment which shows that, despite identical NADH standard curves, lysis of human PBMCs in methanol leads to loss of measurable G6PDH activity when compared to NP-40 lysis.
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提交于 Oct 24 2014