Application
Western blot
Sample
Human Cell lysate - whole cell (THP-1 cells)
Loading amount
10 µg
Specification
THP-1 cells
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Dilution
1/200
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% BSA PBS-Tween (0.1%)
Secondary antibody
Name
Non-Abcam antibody was used: Goat Anti-Mouse IgG (H+L chain specific)
Host species: Goat
Clonality: Polyclonal
Conjugation: Alkaline Phosphatase
Host species: Goat
Clonality: Polyclonal
Conjugation: Alkaline Phosphatase
Dilution
1/1000
Detection
Detection method
ECF
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: light one at predict kDa Non-specific: multiple light non-s kDa
Positive control
THP-1 cytosolic extract
Negative control
THP-1 nucelar Extract
Additional data
Additional Notes
Used Optitran BA-S 85 Nitrocellulose
lane 1. THP-1 Nuclear Cell Extract
lane 2. THP-1 Cytosolic Cell Extract
lane 1. THP-1 Nuclear Cell Extract
lane 2. THP-1 Cytosolic Cell Extract
Abcam response
Thank you for submitting you honest feedback. We always encourage customers to send their results back to us, whether positive or negative, and we make all product information available to researchers.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Jan 08 2008