Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Pancreas)
Specification
Pancreas
Fixative
Paraformaldehyde
Antigen retrieval step
None
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Other product details
Dilution
1/200
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: PBS, 2% Serum
Secondary antibody
Name
Non-Abcam antibody was used: TexasRed, Donkey @ mouse IgG (H+L) dianova
Host species: Donkey
Clonality: Polyclonal
Conjugation: Texas Red ®
Host species: Donkey
Clonality: Polyclonal
Conjugation: Texas Red ®
Dilution
1/250
Additional data
Additional Notes
1µm sections,
blue-> nuclear dye DAPI
green->insulin
red-> glucagon
60x magni
blue-> nuclear dye DAPI
green->insulin
red-> glucagon
60x magni
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mr. Thomas Schöppe
Verified customer
提交于 Jul 15 2011