Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Sample
Human Cell (A549)
Specification
A549
Permeabilization
No
Fixative
Formaldehyde
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: 1% Donkey serum in PBST
Dilution
1/50
Secondary antibody
Name
Non-Abcam antibody was used: Donkey-anti-Ms
Host species: Donkey
Clonality: Polyclonal
Conjugation: DyLight 594
Host species: Donkey
Clonality: Polyclonal
Conjugation: DyLight 594
Dilution
1/200
Additional data
Additional Notes
Red-Giantin blue - nucleus (DAPI)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Armen Petrosyan
Verified customer
提交于 Oct 29 2014