Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acd
Permeabilization
No
Specification
Brain
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative
Formaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: TBS/BSA/azide
Dilution
1/50
Secondary antibody
Dilution
1/250
Name
Non-Abcam antibody was used: anti Rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Additional data
Additional Notes
Submitted coronal composite image ( upper of C.putamen, lower of region below the lateral ventricle called, I believe, the bed nucleus of the tria terminalis) shows a diffuse,moderate positivity in the neuropil of the grey mattter, with occasional neuronal cell body positivity, of a similar intensity.
To me. the positivty appears indistinct ( compare with results for ab125457)
All other regions appeared unremarkable.
NB: in the lower image the anterior commisure is visibe at the lower left.
Abcam response
Many thanks for your feedback in rat IHC-P for ab85104; we invite others with expertise on this target to make comment,
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Carl Hobbs
Verified customer
提交于 Jan 28 2013