Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (fetal ovary)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM citrate buffer
Permeabilization
Yes - 0.05% Tween 20 and 0.1% Triton X-100 in blocking serum
Specification
fetal ovary
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Bouin's fixative
Other product details
Incubation time
1 hour(s) and 30 minute(s) · Temperature: 25°C · Diluent: 1% BSA and 0.5% Triton X-100 in 1XPBS
Dilution
1/200
Secondary antibody
Dilution
1/200
Name
Non-Abcam antibody was used: Alexa Flour 488 goat anti-rat IgG(H+L)
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Additional data
Additional Notes
Using the Thermo sequenza immnostaing system to perform the assay
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提交于 Dec 06 2012