Anti-GCN1抗体(ab86139)
Key features and details
- Rabbit polyclonal to GCN1
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-GCN1抗体
参阅全部 GCN1 一抗 -
描述
兔多克隆抗体to GCN1 -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Human
预测可用于: Rabbit, Horse, Cow, Pig, Chimpanzee, Gorilla, Bat, Elephant -
免疫原
Synthetic peptide corresponding to Human GCN1.
Database link: Q92616 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab86139于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (1) |
1/2000 - 1/10000. Predicted molecular weight: 293 kDa.
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IP |
Use at 2-5 µg/mg of lysate.
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说明 |
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WB
1/2000 - 1/10000. Predicted molecular weight: 293 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
靶标
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功能
Acts as a positive activator of the EIF2AK4/GCN2 protein kinase activity in response to amino acid starvation. Forms a complex with EIF2AK4/GCN2 on translating ribosomes; during this process, GCN1 seems to act as a chaperone to facilitate delivery of uncharged tRNAs that enter the A site of ribosomes to the tRNA-binding domain of EIF2AK4/GCN2, and hence stimulating EIF2AK4/GCN2 kinase activity. Participates in the repression of global protein synthesis and in gene-specific mRNA translation activation, such as the transcriptional activator ATF4, by promoting the EIF2AK4/GCN2-mediated phosphorylation of eukaryotic translation initiation factor 2 (eIF-2-alpha/EIF2S1) on 'Ser-52', and hence allowing ATF4-mediated reprogramming of amino acid biosynthetic gene expression to alleviate nutrient depletion. -
组织特异性
Ubiquitously expressed (PubMed:9039502). Expressed in skeletal muscules, ovary and testis (PubMed:9234705). -
序列相似性
Belongs to the GCN1 family.
Contains 47 HEAT repeats. -
细胞定位
Cytoplasm. Associates with ribosomes in undifferentiated neuroblastoma cells and increases after neuronal differentiation. - Information by UniProt
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数据库链接
- Entrez Gene: 10985 Human
- Omim: 605614 Human
- SwissProt: Q92616 Human
- Unigene: 298716 Human
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别名
- eIF-2-alpha kinase activator GCN1 antibody
- GCN1 (general control of amino acid synthesis yeast homolog) like antibody
- GCN1 (general control of amino acid synthesis 1 yeast) like 1 antibody
see all
图片
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All lanes : Anti-GCN1 antibody (ab86139) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 293 kDa
Observed band size: 293 kDa
Exposure time: 30 seconds -
Detection of Human GCN1 by Immunoprecipitation, using ab86139 at 3µg/mg lysate. Image shows immunoprecipitated GCN1 detected with post IP WB, loading 20% of IP and using HeLa whole cell lysate at 1mg, with ab86139 at 1 µg/ml in lane 1 and control IgG in lane 2. Detection: Chemiluminescence with an exposure time of 30 seconds.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab86139 被引用在 5 文献中.
- Furnish M et al. MIRO2 Regulates Prostate Cancer Cell Growth via GCN1-Dependent Stress Signaling. Mol Cancer Res 20:607-621 (2022). PubMed: 34992146
- Liu J et al. Inducible Systemic Gcn1 Deletion in Mice Leads to Transient Body Weight Loss upon Tamoxifen Treatment Associated with Decrease of Fat and Liver Glycogen Storage. Int J Mol Sci 23:N/A (2022). PubMed: 35328622
- Pinch M et al. General control nonderepressible 1 interacts with cationic amino acid transporter 1 and affects Aedes aegypti fecundity. Parasit Vectors 15:383 (2022). PubMed: 36271393
- Kaplan A et al. Small-Molecule Stabilization of 14-3-3 Protein-Protein Interactions Stimulates Axon Regeneration. Neuron 93:1082-1093.e5 (2017). PubMed: 28279353
- Vartanian S et al. Application of Mass Spectrometry Profiling to Establish Brusatol as an Inhibitor of Global Protein Synthesis. Mol Cell Proteomics 15:1220-31 (2016). PubMed: 26711467