Application
Western blot
Sample
Mouse Tissue lysate - whole (aorta)
Loading amount
12 µg
Specification
aorta
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 27°C
Other product details
Dilution
1/200
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk+ TBST overnight
Secondary antibody
Name
Non-Abcam antibody was used: goat anti rabbit FITC
Host species: Goat
Clonality: Polyclonal
Conjugation: Fluorescein
Host species: Goat
Clonality: Polyclonal
Conjugation: Fluorescein
Dilution
1/16000
Detection
Detection method
CDP-star
Exposure
20 minute(s) and 0 second(s)
Bands
Specific: 45 kDa
Positive control
No positive control
Negative control
No negative control
Additional data
Additional Notes
Ternary Antibodies were anti FITC-AP, 1:20000 dilution in block, 30 minutes incubation
Image was taken with AlphaInnotech camera (20 minutes exposure time)
Image was taken with AlphaInnotech camera (20 minutes exposure time)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Oct 29 2010