Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (cervical tumor and immortalized keratinocytes)
Specification
cervical tumor and immortalized keratinocytes
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.25% Triton X-100 / PBS
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Other product details
Dilution
1/1000
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% BSA / PBS
Secondary antibody
Name
Non-Abcam antibody was used: anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Dilution
1/2000
Additional data
Additional Notes
SiHa cells (negative control very low Gal-7 mRNA), Imm Kera (positive control high Gal-7 mRNA)
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提交于 Aug 17 2010