重组Anti-Galectin 7抗体[EPR4287] (ab108623)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4287] to Galectin 7
- Suitable for: WB, IP, IHC-P, ICC/IF, Flow Cyt (Intra)
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Galectin 7抗体[EPR4287]
参阅全部 Galectin 7 一抗 -
描述
兔单克隆抗体[EPR4287] to Galectin 7 -
宿主
Rabbit -
经测试应用
适用于: WB, IP, IHC-P, ICC/IF, Flow Cyt (Intra)more details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- IP: HaCaT whole cell lysate. ICC/IF: HaCaT cells; Flow Cyt (intra): HaCaT; WB: Human skin lysate, IHC: Human tonsil tissue.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR4287 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab108623于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000 - 1/10000. Detects a band of approximately 15 kDa (predicted molecular weight: 15 kDa).
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IP |
1/10 - 1/100.
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IHC-P |
1/1500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
1/1000.
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Flow Cyt (Intra) |
1/50.
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说明 |
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WB
1/1000 - 1/10000. Detects a band of approximately 15 kDa (predicted molecular weight: 15 kDa). |
IP
1/10 - 1/100. |
IHC-P
1/1500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/1000. |
Flow Cyt (Intra)
1/50. |
靶标
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功能
Could be involved in cell-cell and/or cell-matrix interactions necessary for normal growth control. Pro-apoptotic protein that functions intracellularly upstream of JNK activation and cytochrome c release. -
组织特异性
Mainly expressed in stratified squamous epithelium. -
序列相似性
Contains 1 galectin domain. -
细胞定位
Cytoplasm. Nucleus. Secreted. May be secreted by a non-classical secretory pathway. - Information by UniProt
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数据库链接
- Entrez Gene: 3963 Human
- Entrez Gene: 653499 Human
- Omim: 600615 Human
- SwissProt: P47929 Human
- Unigene: 558355 Human
- Unigene: 707031 Human
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别名
- Gal-7 antibody
- GAL7 antibody
- Galectin 7B antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling Galectin 7 with purified ab108623 at 1/1500 dilution (0.35 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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Anti-Galectin 7 antibody [EPR4287] (ab108623) at 1/1000 dilution (Purified) + Human skin lysate
Secondary
Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 15 kDa -
Intracellular Flow Cytometry analysis of HaCaT (Human skin keratinocyte) cells labeling Galectin 7 with Purified ab108623 at 1/50 dilution (10 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488 ,ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
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Immunocytochemistry analysis of HaCaT (Human skin keratinocyte) cells labeling Galectin 7 with Purified ab108623 at 1:1000 dilution (0.5 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488,ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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Purified ab108623 at 1/50 dilution (2µg) immunoprecipitating Galectin 7 in HaCaT whole cell lysate.
Lane 1 (input): HaCaT (Human skin keratinocyte) whole cell lysate 10µg
Lane 2 (+): ab108623 + HaCaT whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab108623 in HaCaT whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 15 kDa
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab108623 被引用在 6 文献中.
- Umayahara T et al. Protective role of Galectin-7 for skin barrier impairment in atopic dermatitis. Clin Exp Allergy N/A:N/A (2020). PubMed: 32474952
- Yang CF et al. Loss of GPNMB Causes Autosomal-Recessive Amyloidosis Cutis Dyschromica in Humans. Am J Hum Genet 102:219-232 (2018). PubMed: 29336782
- Zhao Y et al. Expression of galectin-7 in vulvar lichen sclerosus and its effect on dermal fibroblasts. Oncol Lett 16:2559-2564 (2018). PubMed: 30013650
- Guo JP & Li XG Galectin-7 promotes the invasiveness of human oral squamous cell carcinoma cells via activation of ERK and JNK signaling. Oncol Lett 13:1919-1924 (2017). WB ; Human . PubMed: 28454344
- Wang J et al. High expression of galectin-7 associates with poor overall survival in patients with non-metastatic clear-cell renal cell carcinoma. Oncotarget 7:41986-41995 (2016). PubMed: 27259255
- Jiang Y et al. Clinical significance of galectin-7 in vulvar squamous cell carcinoma. Oncol Lett 10:3826-3831 (2015). WB . PubMed: 26788216