重组Anti-GTPBP4/NOG1抗体[EPR3500] (ab92342)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3500] to GTPBP4/NOG1
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
-
产品名称
Anti-GTPBP4/NOG1抗体[EPR3500]
参阅全部 GTPBP4/NOG1 一抗 -
描述
兔单克隆抗体[EPR3500] to GTPBP4/NOG1 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human GTPBP4/NOG1 aa 50-150. The exact sequence is proprietary.
-
阳性对照
- Human fetal kidney, fetal brain, Human placenta and Human testis tissue lysates; Human kidney tissue.
-
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
-
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR3500 -
同种型
IgG -
研究领域
相关产品
-
Alternative Versions
-
Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab92342于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | ||
IHC-P |
说明 |
---|
IHC-P: 1/250 - 1/500. Antigen retrieval is recommended.
IP: 1/50.
WB: 1/2000 - 1/5000. Detects a band of approximately 80 kDa (predicted molecular weight: 74 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
靶标
-
功能
Involved in the biogenesis of the 60S ribosomal subunit. -
序列相似性
Belongs to the GTP1/OBG family. NOG subfamily. -
细胞定位
Nucleus > nucleolus. - Information by UniProt
-
数据库链接
- Entrez Gene: 23560 Human
- SwissProt: Q9BZE4 Human
- Unigene: 215766 Human
-
别名
- Chronic renal failure gene protein antibody
- CRFG antibody
- G protein binding protein CRFG antibody
see all
图片
-
All lanes : Anti-GTPBP4/NOG1 antibody [EPR3500] (ab92342) at 1/2000 dilution
Lane 1 : Human fetal kidney tissue lysate
Lane 2 : Human fetal brain tissue lysate
Lane 3 : Human placenta tissue lysate
Lane 4 : Human testis tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 74 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted? -
ab92342 at 1/250 dilution staining GTPBP4/NOG1 in Human kidney by Immunohistochemistry, Paraffin-embedded tissue. The use of an HRP/AP polymerized antibody is recommended for a secondary antibody.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
数据表及文件
-
SDS download
-
Datasheet download
文献 (2)
ab92342 被引用在 2 文献中.
- Shigeoka T et al. On-Site Ribosome Remodeling by Locally Synthesized Ribosomal Proteins in Axons. Cell Rep 29:3605-3619.e10 (2019). PubMed: 31825839
- Li L et al. GTPBP4 Promotes Gastric Cancer Progression via Regulating P53 Activity. Cell Physiol Biochem 45:667-676 (2018). PubMed: 29408813