Application
Western blot
Sample
Mouse Cell lysate - whole cell (Hela,HEK293, fibroblasts, ES cells)
Gel Running Conditions
Reduced Denaturing (Gradientgel 4-12)
Loading amount
10 µg
Specification
Hela,HEK293, fibroblasts, ES cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Incubation time
14 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 3% BSA in PBS Tween 0.1
Dilution
1/500
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: NA934V
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
Immobilon
Exposure
30 minute(s) and 0 second(s)
Bands
Specific: around 120 kDa
Positive control
H3 loading control (see panel B)
Human extracts, lanes 1 and 2 were specific band was observed
Additional data
Additional Notes
I offered to test this antibody in mouse samples (murine samples of different developmental stages are in lanes 3-6 these extracts show intact proteins by commassie and give positive signals with other antibodies) Loading control provided: H3
Lane 1 HeLa
Lane 2 Hek293
Lane 3 Mouse adult fibroblasts
Lane 4 Mouse embryonic fibroblasts
Lane 5 ES cells Pgk12.1
Lane 6 ES cells E14
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Oct 14 2013