Anti-GTF2IRD1抗体[GTF5I102] (ab51524)

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概述

  • 产品名称
    Anti-GTF2IRD1抗体[GTF5I102]
    参阅全部 GTF2IRD1 一抗
  • 描述
    小鼠单克隆抗体[GTF5I102] to GTF2IRD1
  • 宿主
    Mouse
  • 经测试应用
    适用于: WB, ELISA, ICC, IPmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment (internal region).

  • 阳性对照
    • whole cell lysate from HeLa cells transfected with GTF2IRD1 expression construct. RIPA lysate of HeLa cells transfected with GTF2IRD1.

性能

应用

Our Abpromise guarantee covers the use of ab51524 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/50. Detects a band of approximately 130 kDa (predicted molecular weight: 106 kDa).
ELISA Use at an assay dependent dilution.
ICC 1/10.
IP Use at 10 µg/mg of lysate.

靶标

  • 功能
    May be a transcription regulator involved in cell-cycle progression and skeletal muscle differentiation. May repress GTF2I transcriptional functions, by preventing its nuclear residency, or by inhibiting its transcriptional activation. May contribute to slow-twitch fiber type specificity during myogenesis and in regenerating muscles. Binds troponin I slow-muscle fiber enhancer (USE B1). Binds specifically and with high affinity to the EFG sequences derived from the early enhancer of HOXC8.
  • 组织特异性
    Highly expressed in adult skeletal muscle, heart, fibroblast, bone and fetal tissues. Expressed at lower levels in all other tissues tested.
  • 疾病相关
    Note=GTF2IRD1 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. Haploinsufficiency of GTF2IRD1 may be the cause of certain cardiovascular and musculo-skeletal abnormalities observed in the disease.
  • 序列相似性
    Belongs to the TFII-I family.
    Contains 5 GTF2I-like repeats.
  • 发展阶段
    Highly expressed in developing and regenerating muscles, at the time of myofiber diversification.
  • 结构域
    The N-terminal half may have an activating activity.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
    • 别名
      • CREAM1 antibody
      • General transcription factor II I repeat domain containing protein 1 antibody
      • General transcription factor II-I repeat domain-containing protein 1 antibody
      • General transcription factor III antibody
      • GT2D1_HUMAN antibody
      • GTF2I repeat domain containing protein 1 antibody
      • GTF2I repeat domain-containing protein 1 antibody
      • GTF2IRD1 antibody
      • GTF3 antibody
      • Muscle TFII I repeat domain-containing protein 1 antibody
      • Muscle TFII-I repeat domain-containing protein 1 antibody
      • MUSTRD1 antibody
      • MusTRD1/BEN antibody
      • RBAP2 antibody
      • Slow muscle fiber enhancer binding protein antibody
      • Slow-muscle-fiber enhancer-binding protein antibody
      • USE B1 binding protein antibody
      • USE B1-binding protein antibody
      • WBSCR11 antibody
      • WBSCR12 antibody
      • Williams Beuren syndrome chromosome region 11 protein antibody
      • Williams-Beuren syndrome chromosomal region 11 protein antibody
      • Williams-Beuren syndrome chromosomal region 12 protein antibody
      see all

    图片

    • Western blot - Anti-GTF2IRD1 antibody [GTF5I102] (ab51524)
      Western blot - Anti-GTF2IRD1 antibody [GTF5I102] (ab51524)
      Anti-GTF2IRD1 antibody [GTF5I102] (ab51524) at 1/50 dilution + Whole cell lysate from HeLa cells transfected with GTF2IRD1 expression construct at 50 µg

      Secondary
      Mouse IgG antibody at 1/2500 dilution

      Developed using the ECL technique.

      Predicted band size: 106 kDa
      Observed band size: 130 kDa (why is the actual band size different from the predicted?)

    • Immunoprecipitation - Anti-GTF2IRD1 antibody [GTF5I102] (ab51524)
      Immunoprecipitation - Anti-GTF2IRD1 antibody [GTF5I102] (ab51524)
      ab51524 used at 5µg/500µg RIPA lysate of HeLa cells transfected with GTF2IRD1. Western blot was labelled with ab51524 at 1/50 dilution followed by Mouse IgG antibody at 1/2500 dilution. Developed using the ECL technique.
    • Immunocytochemistry - Anti-GTF2IRD1 antibody [GTF5I102] (ab51524)
      Immunocytochemistry - Anti-GTF2IRD1 antibody [GTF5I102] (ab51524)
      HeLa cells stained with ab51524 at 1/10 dilution followed by Alexa Fluor 488 Goat Anti Mouse IgG at 1/200 dilution. Nuclear stain: Hoescht 33342

    文献

    ab51524 has not yet been referenced specifically in any publications.

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