Anti-GST3 / GST pi抗体(ab53943)
Key features and details
- Goat polyclonal to GST3 / GST pi
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-GST3 / GST pi抗体
参阅全部 GST3 / GST pi 一抗 -
描述
山羊多克隆抗体to GST3 / GST pi -
宿主
Goat -
特异性
ab53943 is specific for GST pi. -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Full length native protein (purified) GST3/ GST pi (Rat)
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.7% BSA -
Concentration information loading...
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纯度
IgG fraction -
纯化说明
IgG fraction was prepared from the antisera using the caprylic acid precipitation method. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab53943于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | (2) |
1/3000. Detects a band of approximately 31 kDa (predicted molecular weight: 23 kDa).
|
IHC-P |
1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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说明 |
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WB
1/3000. Detects a band of approximately 31 kDa (predicted molecular weight: 23 kDa). |
IHC-P
1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. -
序列相似性
Belongs to the GST superfamily. Pi family.
Contains 1 GST C-terminal domain.
Contains 1 GST N-terminal domain. - Information by UniProt
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数据库链接
- Entrez Gene: 2950 Human
- Omim: 134660 Human
- SwissProt: P09211 Human
- Unigene: 523836 Human
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别名
- Deafness antibody
- Deafness X-linked 7 antibody
- DFN7 antibody
see all
图片
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Amido Black stain and Western blot analysis of GST-π antibody.
Lane 1. Molecular weight marker.
Lane 2. Purified GST-π
A 1:2000 dilution is recommended for ECL method with overnight incubation at room temperature. -
IHC image of ab53943 staining in human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab53943, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
数据表及文件
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SDS download
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Datasheet download
文献 (17)
ab53943 被引用在 17 文献中.
- Zhang M et al. Ten-eleven translocation 1 mediated-DNA hydroxymethylation is required for myelination and remyelination in the mouse brain. Nat Commun 12:5091 (2021). PubMed: 34429415
- Cheng Y et al. Erzhu Jiedu decoction ameliorates liver precancerous lesions in a rat model of liver cancer. J Cancer 11:7302-7311 (2020). PubMed: 33193894
- Wu C et al. Long Noncoding RNA Plasmacytoma Variant Translocation 1 Regulates Cisplatin Resistance via miR-3619-5p/TBL1XR1 Axis in Gastric Cancer. Cancer Biother Radiopharm N/A:N/A (2020). PubMed: 32407172
- Uzuncakmak TK et al. Comparison of the tissue expressions of glutathione S transferase isoenzymes among patients with morphea and healthy controls: A preliminary study. Dermatol Ther 33:e14363 (2020). PubMed: 33002252
- Cheng Y et al. Knockdown Of lncRNA NCK-AS1 Regulates Cisplatin Resistance Through Modulating miR-137 In Osteosarcoma Cells. Onco Targets Ther 12:11057-11068 (2019). PubMed: 31908475