Application
Western blot
Sample
Escherichia coli Purified protein (Recombinant protein purified from E. coli XL1-Blue)
Loading amount
10 µg
Specification
Recombinant protein purified from E. coli XL1-Blue
Gel Running Conditions
Reduced Denaturing (12 % PAGE gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/2000
Incubation time
3 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: Tris-Saline-Tween with 5% milk
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-Rabbit IgG HRP-conjugated
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/20000
Detection
Detection method
SuperSignal West Pico Chemiluminescent
Exposure
5 second(s)
Bands
Specific: 26, 80 kDa
Positive control
Lane one is purified GST from E. coli and lane to os GST-tagged p53 purified from E. coli.
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提交于 Jun 08 2012