GST Activity Assay试剂盒(Fluorometric) (ab65325)
Key features and details
- Assay type: Enzyme activity
- Detection method: Fluorescent
- Platform: Microplate reader
- Assay time: 2 hr
- Sample type: Cell Lysate, Other biological fluids, Plasma, Purified protein, Saliva, Serum, Tissue Lysate, Urine
- Sensitivity: 0.5 mU/well
概述
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产品名称
GST Activity Assay试剂盒(Fluorometric)
参阅全部 GST 试剂盒 -
检测方法
Fluorescent -
样品类型
Saliva, Urine, Serum, Plasma, Other biological fluids, Cell Lysate, Purified protein, Tissue Lysate -
检测类型
Enzyme activity -
灵敏度
< 0.5 mU/well -
检测时间
2h 00m -
产品概述
Abcam's GST Activity Assay Kit (Fluorometric) provides a simple, fluorescence-based in vitro assay for detecting the GST activity using fluorescence plate reader. The assay utilizes monochlorobimane (MCB), a dye that reacts with glutathione. The free form of MCB is almost nonfluorescent, whereas the dye fluoresces in blue (Ex/Em = 380/461 nm) after reaction with glutathione catalysed by GST. The change in fluorescence overtime allows for an easy measurement of the sample GST level.
The kit can detect GST activity in crude cell lysate or purified protein fraction, and also quantitate GST-tagged fusion protein.
Visit our FAQs page for tips and troubleshooting. -
说明
This product is manufactured by BioVision, an Abcam company and was previously called K260 GST Fluorometric Activity Assay Kit. K260-100 is the same size as the 100 test size of ab65325.
Glutathione S-transferase (GST) is a family of enzymes that play an important role in detoxification of xenobiotics. GST catalyzes the formation of the thiol group of glutathione to electrophilic xenobiotics. It utilizes glutathione to scavenge potentially toxic compounds including those produced as a result of oxidative stress and is part of the defense mechanism against the mutagenic, carcinogenic and toxic effects of such compounds.
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平台
Microplate reader
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 100 tests GSH Substrate 2 vials GST Assay Buffer 1 x 25ml GST Enzyme Standard 1 x 10µl MCB Probe 1 x 200µl -
研究领域
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相关性
Glutathione S-transferase (GST) is a family of enzymes that play an important role in detoxification of xenobiotics. GST catalyzes the formation of the thiol group of glutathione to electrophilic xenobiotics. It utilizes glutathione to scavenge potentially toxic compounds including those produced as a result of oxidative stress and is part of the defense mechanism against the mutagenic, carcinogenic and toxic effects of such compounds. Based on their biochemical, immunological, and structural properties, the soluble human GSTs are categorized into 4 main classes: alpha, mu, pi, and theta. -
细胞定位
Cytoplasmic -
别名
- Glutathione S transferase A1
- Glutathione S transferase A2
- GST class alpha 1
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相关产品
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Related Products
图片
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab65325 被引用在 2 文献中.
- Martens GA et al. Elevated antioxidant defence in the brain of deep-diving pinnipeds. Front Physiol 13:1064476 (2022). PubMed: 36589435
- Plieskatt JL et al. Advances in vaccines against neglected tropical diseases: enhancing physical stability of a recombinant hookworm vaccine through biophysical and formulation studies. Hum Vaccin Immunother 8:765-76 (2012). PubMed: 22495115