重组HRP Anti-GSK3 beta抗体[Y174] (ab196911)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [Y174] to GSK3 beta
- Suitable for: WB
- Knockout validated
- Reacts with: Human
- Conjugation: HRP
Related conjugates and formulations
概述
-
产品名称
HRP Anti-GSK3 beta抗体[Y174]
参阅全部 GSK3 beta 一抗 -
描述
HRP兔单克隆抗体[Y174] to GSK3 beta -
宿主
Rabbit -
偶联物
HRP -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
阳性对照
- WB: A431 whole cell lysate.
-
常规说明
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
存储溶液
pH: 7.40
Preservative: 0.1% Proclin 300 Solution
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading...
-
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
Y174 -
同种型
IgG -
研究领域
相关产品
-
Alternative Versions
- Anti-GSK3 beta antibody [Y174] - BSA and Azide free (ab183177)
- Alexa Fluor® 647 Anti-GSK3 beta antibody [Y174] (ab196910)
- Alexa Fluor® 488 Anti-GSK3 beta antibody [Y174] (ab197236)
- Alexa Fluor® 594 Anti-GSK3 beta antibody [Y174] (ab201737)
- Alexa Fluor® 555 Anti-GSK3 beta antibody [Y174] (ab201738)
- PE Anti-GSK3 beta antibody [Y174] (ab210619)
- Anti-GSK3 beta antibody [Y174] (ab32391)
-
Isotype control
-
Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab196911于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/5000. Detects a band of approximately 38 kDa (predicted molecular weight: 46 kDa).
|
说明 |
---|
WB
1/5000. Detects a band of approximately 38 kDa (predicted molecular weight: 46 kDa). |
靶标
-
功能
Participates in the Wnt signaling pathway. Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN. Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA. Phosphorylates MUC1 in breast cancer cells, and decreases the interaction of MUC1 with CTNNB1/beta-catenin. Phosphorylates CTNNB1/beta-catenin. Phosphorylates SNAI1. Plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. Prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization. Phosphorylates MACF1 and this phosphorylation inhibits the binding of MACF1 to microtubules which is critical for its role in bulge stem cell migration and skin wound repair. -
组织特异性
Expressed in testis, thymus, prostate and ovary and weakly expressed in lung, brain and kidney. -
序列相似性
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. GSK-3 subfamily.
Contains 1 protein kinase domain. -
翻译后修饰
Phosphorylated by AKT1 and ILK1. Activated by phosphorylation at Tyr-216. -
细胞定位
Cytoplasm. Nucleus. Cell membrane. The phosphorylated form shows localization to cytoplasm and cell membrane. The MEMO1-RHOA-DIAPH1 signaling pathway controls localization of the phosophorylated form to the cell membrane. - Information by UniProt
-
数据库链接
- Entrez Gene: 2932 Human
- Entrez Gene: 56637 Mouse
- Omim: 605004 Human
- SwissProt: P49841 Human
- SwissProt: Q9WV60 Mouse
- Unigene: 445733 Human
- Unigene: 394930 Mouse
-
别名
- Glycogen Synthase Kinase 3 Beta antibody
- Glycogen synthase kinase-3 beta antibody
- GSK 3 beta antibody
see all
图片
-
All lanes : HRP Anti-GSK3 beta antibody [Y174] (ab196911) at 1/5000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : GSK3B knockout HAP1 whole cell lysate
Lysates/proteins at 20 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 46 kDa
Observed band size: 46 kDa
Exposure time: 20 minutesab196911 was shown to recognize GSK3 beta in wild-type HAP1 cells as signal was lost at the expected MW in GSK3B knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and GSK3B knockout samples were subjected to SDS-PAGE. Ab196911 and ab184095 (Mouse monoclonal [mAbcam 9484] to GAPDH - Loading Control (Alexa Fluor® 680) loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/1000 dilution respectively. The loading control was imaged using the Licor Odyssey CLx prior to blots being developed with ECL technique.
-
HRP Anti-GSK3 beta antibody [Y174] (ab196911) at 1/5000 dilution + A431 (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 46 kDa
Observed band size: 38 kDa why is the actual band size different from the predicted?
Exposure time: 2 secondsThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab196911 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
数据表及文件
-
SDS download
-
Datasheet download
Certificate of Compliance
文献 (0)
ab196911 尚未被引用在任何文献中。