Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse embryonic stem cells)
Gel Running Conditions
Reduced Denaturing (Bis-Tris from Invitrogen 4-12%)
Loading amount
18 µg
Specification
Mouse embryonic stem cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/1000
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: anti rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL plus
Exposure
17 minute(s) and 0 second(s)
Bands
Specific: 25 kDa Non-specific: 53 kDa
Additional data
Additional Notes
17 min exposition
Abcam response
Thank you for testing ab109024 in an untested species (mouse). We welcome any additional feedbacks in mouse by participation to our AbTrial program: www.abcam.com/abtrial.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Maud de Dieuleveult
Verified customer
提交于 Jul 04 2017