Anti-GRPR抗体(ab39883)
Key features and details
- Rabbit polyclonal to GRPR
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-GRPR抗体
参阅全部 GRPR 一抗 -
描述
兔多克隆抗体to GRPR -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Chicken -
免疫原
Synthetic peptide conjugated to KLH derived from within residues 300 - 400 of Human GRPR.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab39883于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (1) |
Use a concentration of 1 µg/ml. Detects a band of approximately 53 kDa (predicted molecular weight: 43 kDa).
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说明 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 53 kDa (predicted molecular weight: 43 kDa). |
靶标
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功能
Receptor for gastrin releasing peptide (GRP). This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. -
组织特异性
Highly expressed in pancreas. Also expressed in stomach, adrenal cortex and brain. -
序列相似性
Belongs to the G-protein coupled receptor 1 family. -
细胞定位
Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 2925 Human
- Entrez Gene: 14829 Mouse
- Entrez Gene: 24938 Rat
- Omim: 305670 Human
- SwissProt: P30550 Human
- SwissProt: P21729 Mouse
- SwissProt: P52500 Rat
- Unigene: 567282 Human
see all -
别名
- BB2 antibody
- Bombesin BB2 receptor antibody
- bombesin receptor 2 antibody
see all
图片
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All lanes : Anti-GRPR antibody (ab39883) at 1 µg/ml
Lane 1 : Brain (Human) Membrane Lysate
Lane 2 : MDA MB 361 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 3 : Caco-2 whole cell lysate (ab3950)
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 43 kDa
Observed band size: 53 kDa why is the actual band size different from the predicted?
This protein is known to be glycosylated which could explain the difference between the predicted molecular weight of 43kDa according to Swiss Prot and the observed molecular weight of 53kDa.
数据表及文件
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SDS download
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Datasheet download
文献 (8)
ab39883 被引用在 8 文献中.
- Acton D et al. Spinal Neuropeptide Y1 Receptor-Expressing Neurons Form an Essential Excitatory Pathway for Mechanical Itch. Cell Rep 28:625-639.e6 (2019). PubMed: 31315043
- Wang XF et al. Gastrin-releasing peptide receptor gene silencing inhibits the development of the epithelial-mesenchymal transition and formation of a calcium oxalate crystal in renal tubular epithelial cells in mice with kidney stones via the PI3K/Akt signaling pathway. J Cell Physiol 234:1567-1577 (2019). PubMed: 30144073
- Du L et al. Spinal IL-33/ST2 signaling mediates chronic itch in mice through the astrocytic JAK2-STAT3 cascade. Glia 67:1680-1693 (2019). PubMed: 31087583
- Matsumoto Y et al. Gastrin-Releasing Peptide Is Involved in the Establishment of Allergic Rhinitis in Mice. Laryngoscope 128:E377-E384 (2018). PubMed: 30151920
- Pu F et al. GRPR-targeted Protein Contrast Agents for Molecular Imaging of Receptor Expression in Cancers by MRI. Sci Rep 5:16214 (2015). PubMed: 26577829
- Pereira PJ et al. GRPR/PI3K?: Partners in Central Transmission of Itch. J Neurosci 35:16272-81 (2015). PubMed: 26658875
- Santos J et al. Uncovering potential downstream targets of oncogenic GRPR overexpression in prostate carcinomas harboring ETS rearrangements. Oncoscience 2:497-507 (2015). WB . PubMed: 26097883
- Tattersall M et al. Expression of gastrin-releasing peptide is increased by prolonged stretch of human myometrium, and antagonists of its receptor inhibit contractility. J Physiol 590:2081-93 (2012). WB ; Human . PubMed: 22411014