Application
Western blot
Sample
Rat Tissue lysate - whole (liver and heart)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Specification
liver and heart
Blocking step
Milk as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Diluent: TBS-T
Dilution
1/200
Secondary antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: Goat
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ecl
Exposure
30 second(s)
Bands
Specific: 100 kDa Non-specific: 70 kDa
Positive control
human cell line
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Mar 23 2015