Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HEK293 EBNA)
Specification
HEK293 EBNA
Fixative
3% Paraformaldehyde
Permeabilization
No
Blocking step
NH4Cl as blocking agent for 15 minute(s) · Concentration: 0.27% · Temperature: 20°C
Other product details
Dilution
1/100
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 37°C · Diluent: PBS-0.05% saponin
Secondary antibody
Name
Non-Abcam antibody was used: donkey-anti-rabbit Cy3
Host species: Donkey
Clonality: Polyclonal
Conjugation: Cy3 ®
Host species: Donkey
Clonality: Polyclonal
Conjugation: Cy3 ®
Dilution
1/400
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Mar 22 2010