Anti-CXCL1/GRO alpha抗体(ab86436)
Key features and details
- Rabbit polyclonal to CXCL1/GRO alpha
- Suitable for: WB
- Reacts with: Mouse, Human, Recombinant fragment
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-CXCL1/GRO alpha抗体
参阅全部 CXCL1/GRO alpha 一抗 -
描述
兔多克隆抗体to CXCL1/GRO alpha -
宿主
Rabbit -
特异性
The immunogen for ab86436 has >90% homology to mouse GRO beta -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Mouse, Human, Recombinant fragment -
免疫原
Synthetic peptide surrounding amino acid 78 of mouse GRO alpha.
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常规说明
This product is manufactured by BioVision, an Abcam company and was previously called 5212 KC Antibody.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 0.5% BSA, 30% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab86436于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 0.5 - 4 µg/ml. Detects a band of approximately 9 kDa (predicted molecular weight: 11 kDa).
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说明 |
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WB
Use a concentration of 0.5 - 4 µg/ml. Detects a band of approximately 9 kDa (predicted molecular weight: 11 kDa). |
靶标
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功能
Has chemotactic activity for neutrophils. May play a role in inflammation and exerts its effects on endothelial cells in an autocrine fashion. In vitro, the processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) show a 30-fold higher chemotactic activity. -
序列相似性
Belongs to the intercrine alpha (chemokine CxC) family. -
翻译后修饰
N-terminal processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) are produced by proteolytic cleavage after secretion from peripheral blood monocytes. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 2919 Human
- Entrez Gene: 14825 Mouse
- Omim: 155730 Human
- SwissProt: P09341 Human
- SwissProt: P12850 Mouse
- Unigene: 708652 Human
- Unigene: 789 Human
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别名
- C-X-C motif chemokine 1 antibody
- Chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha) antibody
- chemokine (C-X-C motif) ligand 1 antibody
see all
图片
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All lanes : Anti-CXCL1/GRO alpha antibody (ab86436) at 0.5 µg/ml
Lane 1 : Jurkat cell lysate
Lane 2 : 3T3 cell lysate
Predicted band size: 11 kDa
Observed band size: 9 kDa why is the actual band size different from the predicted? -
All lanes : Anti-CXCL1/GRO alpha antibody (ab86436) at 0.5 µg/ml
Lane 1 :Recombinant rat CXCL1/GRO alpha protein (ab9773) at 0.1 µg
Lane 2 :Recombinant rat CXCL1/GRO alpha protein (ab9773) at 0.01 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 11 kDa
Exposure time: 1 minute
数据表及文件
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SDS download
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Datasheet download
文献 (37)
ab86436 被引用在 37 文献中.
- Chang HX & Zhao YF Protocatechuic acid as an inhibitor of the JNK/CXCL1/CXCR2 pathway relieves neuropathic pain in CCI rats. Bosn J Basic Med Sci 22:217-228 (2022). PubMed: 34813418
- Saxena S et al. Differential expression profile of CXC-receptor-2 ligands as potential biomarkers in pancreatic ductal adenocarcinoma. Am J Cancer Res 12:68-90 (2022). PubMed: 35141005
- Catar RA et al. Angiogenic Role of Mesothelium-Derived Chemokine CXCL1 During Unfavorable Peritoneal Tissue Remodeling in Patients Receiving Peritoneal Dialysis as Renal Replacement Therapy. Front Immunol 13:821681 (2022). PubMed: 35185912
- Zingariello M et al. Resident Self-Tissue of Proinflammatory Cytokines Rather Than Their Systemic Levels Correlates with Development of Myelofibrosis in Gata1low Mice. Biomolecules 12:N/A (2022). PubMed: 35204735
- Wang Y et al. Gut-Liver Axis: Liver Sinusoidal Endothelial Cells Function as the Hepatic Barrier in Colitis-Induced Liver Injury. Front Cell Dev Biol 9:702890 (2021). PubMed: 34336855