Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Brain - Cerebellum)
Specification
Brain - Cerebellum
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated
Permeabilization
No
Other product details
Dilution
1/3000
Incubation time
1 hour(s) and 0 minute(s) · Diluent: TBS
Secondary antibody
Secondary antibody
None used
Additional data
Additional Notes
Used to investigate respiratory deficiency in 5µm cut sections of FFPE human cerebellum. Sections were cut and mounted on to glass adhesion slides for staining. The antibody stained specifically, identifying both normal neurons as well as those exhibiting respiratory deficiency. In comparison to C1-20 (AB110242) staining was not as intense, and we tend to find more variability with this antibody between IHC runs.
Detection - Menarini Diagnostics X-Cell Plus HRP-Polymer Kit.
SigmaFast DAB tablets were used for the final stage of staining.
Detection - Menarini Diagnostics X-Cell Plus HRP-Polymer Kit.
SigmaFast DAB tablets were used for the final stage of staining.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MS. Philippa Hepplewhite
Verified customer
提交于 Jun 23 2012