Anti-GRC5 / PHF2抗体(ab65771)
Key features and details
- Rabbit polyclonal to GRC5 / PHF2
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-GRC5 / PHF2抗体
参阅全部 GRC5 / PHF2 一抗 -
描述
兔多克隆抗体to GRC5 / PHF2 -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide, corresponding to C terminal amino acids 830-1096 of Human GRC5/ PHF2
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阳性对照
- Nuclear extracts of HeLa cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
Constituent: Whole serum -
Concentration information loading...
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纯度
Whole antiserum -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab65771于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000. Detects a band of approximately 130 kDa (predicted molecular weight: 121 kDa).
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说明 |
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WB
1/1000. Detects a band of approximately 130 kDa (predicted molecular weight: 121 kDa). |
靶标
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功能
Lysine demethylase that demethylates both histones and non-histone proteins. Enzymatically inactive by itself, and becomes active following phosphorylation by PKA: forms a complex with ARID5B and mediates demethylation of methylated ARID5B. Demethylation of ARID5B leads to target the PHF2-ARID5B complex to target promoters, where PHF2 mediates demethylation of dimethylated 'Lys-9' of histone H3 (H3K9me2), followed by transcription activation of target genes. The PHF2-ARID5B complex acts as a coactivator of HNF4A in liver. PHF2 is recruited to trimethylated 'Lys-4' of histone H3 (H3K4me3) at rDNA promoters and promotes expression of rDNA. -
组织特异性
Widely expressed, including in liver (at protein level). -
序列相似性
Belongs to the JHDM1 histone demethylase family. JHDM1D subfamily.
Contains 1 JmjC domain.
Contains 1 PHD-type zinc finger. -
结构域
The PHD-type zinc finger mediates the binding to H3K4me2 and H3K4me3. -
翻译后修饰
Phosphorylated by PKA on specific serine residues, leading to the formation of an active lysine demethylase complex. -
细胞定位
Nucleus > nucleolus. - Information by UniProt
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数据库链接
- Entrez Gene: 5253 Human
- Omim: 604351 Human
- SwissProt: O75151 Human
- Unigene: 211441 Human
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别名
- CENP-35 antibody
- GRC5 antibody
- JHDM1E antibody
see all
图片
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Anti-GRC5 / PHF2 antibody (ab65771) at 1/1000 dilution + Nuclear extracts of HeLa cells.
Predicted band size: 121 kDa
Observed band size: 130 kDa why is the actual band size different from the predicted?
数据表及文件
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Datasheet download
文献 (0)
ab65771 尚未被引用在任何文献中。