Anti-GRB2抗体(ab2234)
Key features and details
- Goat polyclonal to GRB2
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human, Pig
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-GRB2抗体
参阅全部 GRB2 一抗 -
描述
山羊多克隆抗体to GRB2 -
宿主
Goat -
经测试应用
适用于: IHC-P, WBmore details -
种属反应性
与反应: Mouse, Rat, Human, Pig
预测可用于: Cow, Dog -
免疫原
Synthetic peptide corresponding to Human GRB2 aa 206-217 (C terminal).
Sequence:C-PRNYVTPVNRNV
(Peptide available asab22873) -
阳性对照
- Recombinant Human GRB2 protein (ab85917) can be used as a positive control in WB. WB: MOLT4 cell lysate, mouse, rat, human and pig spleen, mouse and rat brain, and human thymus tissue lysates. IHC-P: Human lymph node tissue.
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常规说明
Principal Names - GRB2; growth factor receptor-bound protein 2 Official Gene Symbol - GRB2. GenBank Accession Number – NP_002077. LocusLink ID - 2885. Gene Ontology terms - cell-cell signalling; SH3/SH2 adaptor protein; EGF receptor signalling pathway; RAS protein signal transduction; cell shape and cell size control.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.05% Tris, 0.5% BSA -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
应用 | Ab评论 | 说明 |
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IHC-P |
Use a concentration of 1 - 2 µg/ml.
In paraffin embedded Human Tonsil, stains mantle zone and to a lessser extent germinal centre. |
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WB | (1) |
Use a concentration of 0.05 - 0.15 µg/ml. Predicted molecular weight: 30 kDa.
A 1 hour primary incubation is recommended for this product. |
说明 |
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IHC-P
Use a concentration of 1 - 2 µg/ml. In paraffin embedded Human Tonsil, stains mantle zone and to a lessser extent germinal centre. |
WB
Use a concentration of 0.05 - 0.15 µg/ml. Predicted molecular weight: 30 kDa. A 1 hour primary incubation is recommended for this product. |
靶标
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功能
Adapter protein that provides a critical link between cell surface growth factor receptors and the Ras signaling pathway.
Isoform GRB3-3 does not bind to phosphorylated epidermal growth factor receptor (EGFR) but inhibits EGF-induced transactivation of a RAS-responsive element. Isoform GRB3-3 acts as a dominant negative protein over GRB2 and by suppressing proliferative signals, may trigger active programmed cell death. -
序列相似性
Belongs to the GRB2/sem-5/DRK family.
Contains 1 SH2 domain.
Contains 2 SH3 domains. -
结构域
The SH3 domains mediate interaction with RALGPS1 and SHB. -
细胞定位
Golgi apparatus. - Information by UniProt
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数据库链接
- Entrez Gene: 535298 Cow
- Entrez Gene: 483312 Dog
- Entrez Gene: 2885 Human
- Entrez Gene: 14784 Mouse
- Entrez Gene: 100192436 Pig
- Entrez Gene: 81504 Rat
- Omim: 108355 Human
- SwissProt: P62993 Human
see all -
别名
- Abundant SRC homology antibody
- Adapter protein GRB2 antibody
- ASH antibody
see all
图片
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All lanes : Anti-GRB2 antibody (ab2234) at 0.05 µg/ml
Lane 1 : Mouse brain tissue lysate in RIPA buffer
Lane 2 : Rat brain tissue lysate in RIPA buffer
Lane 3 : Mouse spleen tissue lysate in RIPA buffer
Lane 4 : Rat spleen tissue lysate in RIPA buffer
Lysates/proteins at 35 µg per lane.
Predicted band size: 30 kDa
Observed band size: 26 kDa why is the actual band size different from the predicted?Primary incubation for 1 hour. Detected using chemiluminescence.
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ab2234 (1µg/ml) staining of paraffin embedded Human Lymph Node tissue. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.
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ab2234 negative control showing staining of paraffin embedded Human Lymph Node tissue, with no primary antibody.
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All lanes : Anti-GRB2 antibody (ab2234) at 0.05 µg/ml
Lane 1 : Human spleen tissue lysate in RIPA buffer
Lane 2 : Pig spleen tissue lysate in RIPA buffer
Lysates/proteins at 35 µg per lane.
Predicted band size: 30 kDa
Observed band size: 26 kDa why is the actual band size different from the predicted?Primary incubation for 1 hour. Detected using chemiluminescence.
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Lane 1 : Anti-GRB2 antibody (ab2234) at 0.05 µg/ml
Lane 2 : Anti-GRB2 antibody (ab2234) at 0.05 µg
Lane 1 : Human thymus tissue lysate in RIPA buffer
Lane 2 : MOLT4 cell lysate in RIPA buffer
Lysates/proteins at 35 µg per lane.
Predicted band size: 30 kDa
Observed band size: 26 kDa why is the actual band size different from the predicted?Primary incubation for 1 hour. Detected using chemiluminescence.
数据表及文件
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Datasheet download
文献 (1)
ab2234 被引用在 1 文献中.
- Debattisti V et al. Dysregulation of Mitochondrial Ca2+ Uptake and Sarcolemma Repair Underlie Muscle Weakness and Wasting in Patients and Mice Lacking MICU1. Cell Rep 29:1274-1286.e6 (2019). PubMed: 31665639