Anti-LGN抗体(ab109594)
Key features and details
- Rabbit polyclonal to LGN
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-LGN抗体
参阅全部 LGN 一抗 -
描述
兔多克隆抗体to LGN -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Human
预测可用于: Chimpanzee, Rhesus monkey, Gorilla -
免疫原
Synthetic peptide corresponding to Human LGN aa 627-677. (AAH27732.1)
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阳性对照
- HeLa or 293T whole cell lysate
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab109594于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 77 kDa.
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IP |
Use at 2-5 µg/mg of lysate.
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说明 |
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WB
1/2000 - 1/10000. Predicted molecular weight: 77 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
靶标
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功能
Plays an important role in spindle pole orientation. Interacts and contributes to the functional activity of G(i) alpha proteins. Acts to stabilize the apical complex during neuroblast divisions. -
组织特异性
Ubiquitously expressed. -
疾病相关
Defects in GPSM2 are the cause of deafness autosomal recessive type 82 (DFNB82) [MIM:613557]. DFNB82 is a form of non-syndromic deafness characterized by prelingual, bilateral, severe, sensorineural hearing loss. There are no symptoms of vestibular dysfunction. -
序列相似性
Belongs to the GPSM family.
Contains 4 GoLoco domains.
Contains 8 TPR repeats. -
细胞定位
Cytoplasm. Cytoplasm > cell cortex. Localizes in the cytoplasm in the interphase and at cell periphery in the metaphase. - Information by UniProt
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数据库链接
- Entrez Gene: 29899 Human
- Omim: 609245 Human
- SwissProt: P81274 Human
- Unigene: 584901 Human
- Unigene: 658489 Human
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别名
- DFNB82 antibody
- G protein signalling modulator 2 (AGS3 like C. elegans) antibody
- G protein signalling modulator 2 antibody
see all
图片
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All lanes : Anti-LGN antibody (ab109594) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 77 kDa
Exposure time: 3 minutes -
Detection of LGN by Western Blot of Immunprecipitate.
ab109594 at 1µg/ml staining LGN in HeLa whole cell lysate immunoprecipitated using ab109594 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 30 seconds.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab109594 被引用在 1 文献中.
- Freeman S et al. Proteostasis is essential during cochlear development for neuron survival and hair cell polarity. EMBO Rep 20:e47097 (2019). PubMed: 31321879