Application
Western blot
Sample
Human Cell lysate - whole cell (SW620 Colon)
Gel Running Conditions
Reduced Denaturing (7)
Loading amount
100 µg
Treatment
siRNA - knockdown
Specification
SW620 Colon
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST
Dilution
1/500
Secondary antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: Sigma
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
20 minute(s) and 0 second(s)
Bands
Specific: 100 kDa Non-specific: numerous bands kDa
Positive control
SW620 a positive cell-line transfected with LGR5 siRNA
Additional data
PubMed ID
PMID:23349017: View
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Apr 08 2013