Application
Western blot
Sample
Mouse Cell lysate - whole cell (HEK 293T cell transfected with pGOSR1-EGFP)
Gel Running Conditions
Reduced Denaturing (4-15)
Loading amount
20 µg
Specification
HEK 293T cell transfected with pGOSR1-EGFP
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% BSA
Dilution
1/500
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: Anti Rabbit IgG
Host species: Rabbit
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL+
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 29 kDa
Positive control
HEK 293 transfected with pGOSR1-EGFP
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Apr 25 2013