Anti-GORASP2/GRASP55抗体(ab68713)
Key features and details
- Mouse polyclonal to GORASP2/GRASP55
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
-
产品名称
Anti-GORASP2/GRASP55抗体
参阅全部 GORASP2/GRASP55 一抗 -
描述
小鼠多克隆抗体to GORASP2/GRASP55 -
宿主
Mouse -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human -
免疫原
Recombinant full length protein within Human GORASP2/GRASP55. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. (NP_056345.3, 1 a.a. ~ 452 a.a)
-
阳性对照
- mouse neural stem cell lysate, GORASP2/GRASP55 transfected 293T lysate.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.4
Constituent: PBS -
Concentration information loading...
-
纯度
Protein A purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab68713于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | (1) |
1/500 - 1/1000. Detects a band of approximately 52 kDa (predicted molecular weight: 48 kDa).
|
说明 |
---|
WB
1/500 - 1/1000. Detects a band of approximately 52 kDa (predicted molecular weight: 48 kDa). |
靶标
-
功能
May be involved in assembly and membrane stacking of the Golgi cisternae, and in the process by which Golgi stacks reform after mitotic breakdown. May regulate the intracellular transport and presentation of a defined set of transmembrane proteins, such as transmembrane TGFA. -
序列相似性
Belongs to the GORASP family.
Contains 1 PDZ (DHR) domain. -
翻译后修饰
Myristoylated. Myristoylation is essential for the Golgi targeting.
Palmitoylated.
Phosphorylated in mitotic cells. -
细胞定位
Golgi apparatus membrane. - Information by UniProt
-
数据库链接
- Entrez Gene: 26003 Human
- Omim: 608693 Human
- SwissProt: Q9H8Y8 Human
- Unigene: 431317 Human
-
别名
- AW552058 antibody
- DKFZp434D156 antibody
- FLJ13139 antibody
see all
图片
-
All lanes : Anti-GORASP2/GRASP55 antibody (ab68713) at 1/500 dilution
Lane 1 : GORASP2 / GRASP5 transfected 293T lysate
Lane 2 : Non-transfected 293T lysate
Lysates/proteins at 25 µg per lane.
Secondary
Lane 1 : Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
Lane 2 : Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
Predicted band size: 48 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted? -
Anti-GORASP2/GRASP55 antibody (ab68713) at 1/500 dilution + neural stem cell lysate at 30 µg
Secondary
Peroxidase labeled horse anti-mouse IgG (H+L) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 48 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
Exposure time: 10 minutes
数据表及文件
-
Datasheet download
文献 (1)
ab68713 被引用在 1 文献中.
- Kim YH et al. Secretory autophagy machinery and vesicular trafficking are involved in HMGB1 secretion. Autophagy 17:2345-2362 (2021). PubMed: 33017561