Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Ileum)
Specification
Ileum
Blocking step
BSA as blocking agent for 45 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Formaldehyde
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST+1%BSA
Secondary antibody
Name
Non-Abcam antibody was used: Donkey anti-Goat IgG (H+L) Cross-Adsorbed Secondar
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor 488
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor 488
Additional data
Abcam response
We are sorry the antibody did not work very well on this occasion. ICC/IF is not tested or guaranteed application. The customer was using Abtrial in this case.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Mar 24 2017